Regulatory

Part:BBa_K592003:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-17)

PcpcG2 promoter, ccaR-regulated


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was amplified from Tabor's pJT122 plasmid. Due to the nature of the sequence, optimizing primers proved to be difficult.


Source

Synechocystic sp. PCC6803, Jeffrey Tabor's pJT122 plasmid

References

Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405, 2, 315–324.